By Prof. Dr. U. Trendelenburg (auth.), Prof. Dr. Peter Riederer, Prof. Dr. Moussa B. H. Youdim (eds.)
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Extra resources for Amine Oxidases and Their Impact on Neurobiology: Proceedings of the 4th International Amine Oxidases Workshop, Wurzburg, Federal Republic of Germany, July 7–10, 1990
Imaging striatal dopaminergic neurons Because catecholamines do not penetrate the blood-brain barrier, replacement of dopamine which is deficient in brain of patients with Parkinson's disease requires use of L-DOPA, the amino acid precursor of this neurotransmitter. L-DOPA, usually administered with a peripheral decarboxylase inhibitor, is transported across the blood-brain barrier and is decarboxylated to form dopamine. As indicated above, the decarboxylase is not specific and will decarboxylate a variety of amino acids, including analogues of L-DOPA such as 5- or 6-fluorodopa.
Subfragments used include: the 5' end region (As, nucleotide no. 1-552), the middle region (A9' nucleotide no. 553-1200) and the 3' end region (A10' nucleotide no. 1201-1958) of the MAO-A cDNAs, the 5' end (B1' nucleotide no. 1-602), the middle region (B2' nucleotide no. 603-1434), and the 3' end (B3' nucleotide no. , 1989a). 1 % SDS at 65°C and exposed for two days. Under these conditions, MAO-A and B transcripts do no cross-hybridize. Results We found a 5Kb transcript in most of the fetal tissues examined when the MAO-A cDNA subfragments were used (Fig.
2, lane 3). Discussion We demonstrated the existence of a 5Kb transcript for MAO-A and a 3Kb transcript of MAO-B in most fetal tissues we examined. Under our experimental conditions, the MAO-A 5Kb transcript was not detected in the thymus and heart while the MAO-B 3Kb transcript was not detected in the meninges and thymus. We were able to exclude the possibility that RNA degradation was causing this finding with use of the 28s rDNA probe. Our finding that the placenta had approximately equal concentrations of both 44 J.