By I. F. Wilson, E. M. Gosling (auth.), Ruth M. O’Riordan, Gavin M. Burnell, Mark S. Davies, Neil F. Ramsay (eds.)
Members of the relations Littorinidae are one of the most generally studied gastropod molluscs and the extra questions we solution approximately this team, the extra questions are unavoidably posed. Littorinid study spans different disciplines, from molecular biology, body structure, ecology, systematics and evolutionary biology to dependent anatomical stories. The papers n this quantity mirror the present learn being performed on littorinids, and fall into 3 large topics: systematics, ecology, and toxins reviews.
This ebook is essentially distinctive on the study point, whereas offering priceless details for complex first-degree scholars carrying out learn projects.
Read Online or Download Aspects of Littorinid Biology: Proceedings of the Fifth International Symposium on Littorinid Biology, held in Cork, Ireland, 7–13 September 1996 PDF
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Extra resources for Aspects of Littorinid Biology: Proceedings of the Fifth International Symposium on Littorinid Biology, held in Cork, Ireland, 7–13 September 1996
The lysate was thereafter extracted with an equal volume of phenol. The mixture was gently but persistently shaken during 5-6 minutes and centrifuged at 6000 rpm for 5 minutes. The supernatant was transferred to a new eppendorf tube and an equal volume of a mixture of chloroform and isoamyl alcohol (24: 1) was added. The new mixture was shaken and spun at the same conditions as before. The phenol-chloroform procedure was repeated to thoroughly extract the DNA. Following the extraction we used two volumes of cold 95% ethanol to precipitate the DNA and thereafter it was spun down at 6000 rpm for 5 minutes.
Unpublished). We successfully scored individual embryos of Littorina saxatilis, as described below. When analysing adult snails we extracted DNA from about 2 mg muscle tissue taken from the head and mantle. 1 mg ml- I . When analysing individual embryos we added 10 ttl 5% Chelex 100 solution to one embryo taken directly from the brood pouch of a female, and in this case we used the exact protocol of Sundberg & Andersson (1995). The concentration of extracted DNA from one embryo of L. saxatilis was about 7-8 ng ttl-I, and this was obviously more than enough for the amplification procedure.
1998 Kluwer Academic Publishers. 33 A comparison of different protocols for RAPD analysis of Littorina N. Mikhailova 1,2 & K. Johannesson 2,* Institute of Cytology RAS, St Petersburg 194064, Russia Tjdrno Marine Biological Laboratory, S-452 96 Stromstad, Sweden (* author for correspondence) 1 2 Key words: protocols, RAPD, Littorina saxatilis, L. obtusata, L. fabalis, L. littorea, extracting DNA, amplifying DNA Abstract Randomly amplified polymorphic DNA (RAPD) is a fast and useful method of genome marking that is useful for studies of, for example, parentage, mating patterns, taxonomy of sibling species and intra-specific population genetic structures.